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So;mTMRo;m 3 0 So;m 4 0 MRo;m Y(W|u-N 1.6 Nxxg| ScؚTbNnxovv;m'` Rxxp_NnxlS:NNnx0 YS_Nnx N~YNpCoAۏeQTCAe Nnxy/} xxp_NnxlSbIpYNx TSTba.aT'VvU8hx0TbQv'VvU8hx So;mxxpNnxSv OۏIpYNxTb OTCA[IpYNxv0 30 vTbvWVhv vTb /fǏvϑvSSegcN"s0mZ" """Pb+7 N0 ~ހ4ls^v 1 c6R荜yP[Sm^CR^T5uMOh^ 2 c6R荜ir(Џ 3 :SSSm)\O(u 2bkr^pb ON 4 NvS~T SsQvNS~T Ǐ~TWTSnWvNSegvv;m'`0SsQv'YY/fN"_vsQ.vTv Y|u-Nv]|ov xxg|ov )v 3-xxul1"lv (lWxN"vGlu1"lv Tyr'lSvSNqQNOpvˆ}vov ˆ}vxxvI{0 N0 o }4ls^v|"""2% ,{N WVhv WVh gQ*N4ls^v t$lU_4ls^ u$l4ls^ v$R]4ls^ lU_TR]0ыTR] w$ˆ}v(;m'` vQ-NgsQ.v/ft$ WVhvc6R;NSu(WlU_4ls^,S8huir$\vQYdk0 e^ ^ N0 S8huirWVhv 10 ~P[!jW d~P[/fWVhvOSUSMO [+T g(WR N|_dk gsQvY*N~gWVSc6RMO c6RMO1u/TRP[Td~WV~b0 N*Nd~P[vhQWVcR(WNw vQ-N+TY*N~gWV lU_Nir/fUS*NYzSmRNA d~P[vc6RMOSSWVNirv0 20 ~bWWVT[WWV ~bvgbv SsXq_T\ ck8^N"agN Nh0Y|u㉄vv0 [v^Wv [ N TvuX[sX g N Tvh0YJSsN|v0)ZMZ#"#c"  ""t" "C&l  30 ckcTc (Wl gˆ}v(X[(We WV/fsQ필v ReQˆ}vT WV;m'`_/T dk:Nckc0 (Wl gˆ}vX[(We WV/fhv ReQˆ}vTWVh;m_sQ dk:Nc0 (Wckc-N ˆ}vy[ˆ}v0 (Wc-N ˆ}vy;Gˆ}v0 40 S8huir~gWVhv4yc6R!j_0 c[\O(u ^O;Gˆ}v㉻yDNA0 ;G\O(u ^O;Gˆ}v~TDNA0 P451V22-25 ckc[\O(u ^O[ˆ}v~TDNA0 ;G\O(u ^O[ˆ}v㉻yDNA0 VGr9-1 0hggku 0 P272 Z""v" ""p     50 'YFg̃sN|d~P[ Lacd~P[ ~gV: P453 V22-26 LacZ0LacY0LacA:N~gWV N8nO!k:Nd~WV0/TRP[TWVLacI0 S_~ހQe[irsN|bIPTG X[(We ;Oˆ}vNd~WV~T01uNd~WVN/TRP[ gN[ z^͑S YxNRNAZTv(W-10^R Nb_b_>e'`/TRP[ YTir0 S_~ހQ g[irsN|bIPTG X[(We [irN;Oˆ}v~T 9eS;Oˆ}vga OKNŏNd~WV N㉻y Neg0ُ7hRNAZTv1\N/TRP[~T v^b_b_>e'`/TRP[ YTir N _YlU_LacZYA~gWV0 VGr8-3 0Y[CNi` 0P 285 IPTG_NW--DkxNJSsN|[pa[ir [sN|d~P[Nug:_v[He^ /f:_[ir0\^Z" ""D     %#) &  60 r(lxd~P[trp vlU_c trpd~P[#TrpvTb 8^/f_>ev WVvNirO[sQ ُyc\O(uy:NS;OWvc0 t$E.coli trpd~P[ g5*N~gWV trpE-D-C-B-A0 u$(WtrpEv N8n g N*N:SktrpP-O-L trpL/fNk162bp^R lU_0RmRNA-Nb:NMR[^R [d~P[vlU_wc\O(u0 v$(WgrSO90R:S gtrpRWV x12.5kdv;Oˆ}vNW,NVZSOb__~T0RtrpO0 TrpPNN,S8hWV/TRP[N7h wQ g-10^RT-35^R -10^R[hQMONtrpPKNQ0 E.coli trpd~P[v~bSWVNirvR0 VGr E.coli wQ gTbTya.avR0(WYpe`Q N S g(WW{QW NO^Yna.ae MbSTbNua.a@b_{vv|0 S_~ހQTrpSm^ؚe TrpN;Oˆ}vtrpRWVNir ~T NO[wQ g;m'` N NtrpOWV~T sQlU_0 S_~ހQTrpSm^_NOe ;OGˆ}v NvTrp㉻yQeg O;Oˆ}v1Y;m v^1YSNtrpO~TvR _/TlU_0 VGr\Z"""p*    $   ! 70 trpd~P[vMR[^R trp mRNARP[Ne_YTb (WtrpEWV_YlU_KNMR 'YYpemRNAO\Pbku ُ/fV:NMR[^RtrpL [d~P[cS%cN͑\O(u0 trp mRNAvMR[^RSMR[0 ~gWV N8nwQ g/TRP[ d~WV MR[^R pQP[:S0 mRNA 5 z g162b vQ-N139*NgbMR[^R0MR[^R1u14*Na.avMR[04*NNe:SkT1*NpQP[~bkpgb0 pQP[MON~gWV N8nMR[:Sv~bkOS0V "" M   (." dMR[^Rvyrp t$MR[^RvgN:Sk[+TGC0>\ gN*N+T8*NUv:Sk fgb NOVNv~bkOS0(3:SN4:Sgb~bkOSvS9Y~g) u$1:ST2:Sgb,{N*NS9Y~g vQ-N1:SYN14*Na.avMR[^R-N0 v$3:SN2:S_Nb_bSN*NS9Y~g N S;bk3:SN4:Sb_b~bkS9Y~g0 w$MR[^RRNAxNk14a.avMR[ v^ gN~bk[xP[UGA 24 # hx$MR[^R-N v^RN*NTrp[xP[. 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VGr: ;OTpQ:g6R }6q/f(WlU_4ls^ NۏL FO/f[Nv\O(u:g6R[hQ N T MRc6RlU_vwY Tc6RlU_wYT/f&T~~ NS0 (lWxTbd~P[MR[^R P454h22-2 u^: %N'}c6R WVhe^ ы4ls^L q($  N0 w8hWVhv X " "       rF  F$<1U(   +ech Word.Document.80"Microsoft Word ech0VGr Word.Picture.80"Microsoft Word VGr0VGr Word.Picture.80"Microsoft Word VGr*/ 0`DTimes New Roman |dv 0|( 0D[SOes New Roman |dv 0|( 0 DўSOes New Roman |dv 0|( 00DfNes New Roman |dv 0|( 01@Dёq\{OxNew Roman |dv 0|( 0PD OxNew Roman |dv 0|( 0`D|^WOxNew Roman |dv 0|( 01pDArialNew Roman |dv 0|( 0" ` .  @n?" dd@  @@`` | # ,74-     !oR$ԴrHK$QgQ7.7w&2b$ 3Vtܼ;H&2b$8?Tubq kn2b$t.^}dg $i/22$:fZaK6$H22$k7=RgJMa2c $@7uʚ;2Nʚ;g4CdCdv 0pppp@ <4!d!d` 0,| <4dddd` 0,|  ? %K  ,{ASVz ~ހN"TWVhvc ~ހN"Sbir(N"TϑN"0~ހN"/fN*N[te~NvQ~ v^NX[(W YBgv:g6R ُN:g6R/f(WWVhNirˆ}v(bRNA v\O(u NۏLv0 ͑pir(N"_vvNT| v;m'`v0 fL" "dR!  ,{N ir(N"_vvNT| ~ހN"vW,gSR/f\T{|ir(R+R~eQTꁄvqQ TN"_ N\pey{|vS^lSy{|A~YvRP[0 N TN"_SNǏNSp NsQ.v-Nir vNlS vQ-N N*NsQ.v-Nir/fG-6-P0Nnx0YNpCoA0 N0 |N"NN"vT| 10 |lSb V |~Ǐu ubxxNNnSNnx0xxNNn؏S:Nul Nnx'lS1lSbYNpCoA Tbx0 20 lSb| V ul~xxS:N3-xxul lS:NxxNNn _u:N|0 (W iir0~̃-N xlSbYNpCoA T~YNx_sub%tsx ۏeQTCA 1uIpYNx1ubNnx u|0 RirSOQ eYNx_s YNpCoAۏeQTCA'lS ubCO2TH2O0 x(WRirSOQ_NSNlSb| FOdke_ gvQNegnvir(eEQTCA-Nmv g:gxIpYNx 0 |)R(uS; O`{|ir(Oϑ RXT (W-NNu'YϑnSONn0YNpYNx0-WNx 0ZZ#" #e" ""A""}  erPI 6  .N0 |N"N(lWxN"vsQ| 10 |vRN":N(lWxTbcOxg V | ! Nnx ! -n bNx + IpYNx ُ Nynx ~Ǐl(l\O(uR+RubAla0GluTAsp0 20 u|(lWxvxgSNlSb| Q/fubNnx0 n bNx0%tsx0IpYNxva.a y:Nu|a.a0 Phe0Tyr0Ilr0Lys0TrpI{SubYNpYNpCoA N ubnSO0 Phe0TyrI{u|Sun0 N0 (lWxN"NN"vsQ| (lWxvxgSNg~lSbYNpCoA SN(uNxTƀ>uvTb0 u|a.avxgSNlSbul0 SerSNlSbƀTƀx TbxTuSx0 RirSOQxvM㉧NirYNpCoA N:Na.aTbcOQxg0 {|RP[-NvulSNlS:NNnx ~TCAۏNeklS:NIpYNx0 n bNx ُ NSNlSb(lWx0Z" ""=""k" "9   ) $  2V0 8hxN"N|00(lWxvsQ| 8hx N/f͑vxn0.lnTn0 Ty(lWx   !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~8Root EntrydO)@?@ABCDEFGHIJKLMNOPQRSTUVI 6  .N0 |N"N(lWxN"vsQ| 10 |vRN":N(lWxTbcOxg V | ! Nnx ! -n bNx + IpYNx ُ Nynx ~Ǐl(l\O(uR+RubAla0GluTAsp0 20 u|(lWxvxgSNlSb| Q/fubNnx0 n bNx0%tsx0IpYNxva.a y:Nu|a.a0 Phe0Tyr0Ilr0Lys0TrpI{SubYNpYNpCoA N ubnSO0 Phe0TyrI{u|Sun0 N0 (lWxN"NN"vsQ| (lWxvxgSNg~lSbYNpCoA SN(uNxTƀ>uvTb0 u|a.avxgSNlSbul0 SerSNlSbƀTƀx TbxTuSx0 RirSOQxvM㉧NirYNpCoA N:Na.aTbcOQxg0 {|RP[-NvulSNlS:NNnx ~TCAۏNeklS:NIpYNx0 n bNx ُ NSNlSb(lWx0Z" ""=""k" "9   ) $  2V0 8hxN"N|00(lWxvsQ| 8hx N/f͑vxn0.lnTn0 Ty(lWx YGly 0Asp 0Gln/f8hxvTbMRSO0 gN8hx(Wir(N"-N_N g͑\O(u ATP OSxxWV0 UTP SNUS|lSbY|;mSUS| 0 CTP SNuSxTb0 GTP :Nˆ}v(TbO0L""x.    N0 ir(N"vyrp 10 TCA/f-N_s N"_NSb_bQ~ ;NT|irNnx0YNpCoA0gjx0-n bNx0IpYNx0 20 R0Tb___/fR_v N/f{USvS^0 (WNagN"_-N gNsQ.MOvckS^TS^ __1u$Ny N TvvPS NyvPSckS^ SNyvPSS^0 N|N":NO P421 30 ATP/f(uvϑ}SO YNpCoAۏeQTCAT [hQ'lSubCO20H2O ʑ>ev1uADPUclЏ0&TR 1uNpb__ceS0RhTVsX-N0" "",""I""  &j  $40 R:NTbcO؏SRTϑ ir(N"vW,geu(WNubATP0؏SRT~gUSCQ(uNSOQuirTb0 NADPHNN(uN؏S'`uirTb NADHTFADH2;NR/fǏ|T8TNuATP0 ATPegn1 ^ir4ls^xxS02 ~r iirTIQT~̃vIQTxxS03 |T8Tv'lSxxS0 NADPHegn 1 iirIQT5uP[ O 2 xx b|_ 3 YNpCoA1u~|SOly0R~ހ(e4O gNADHv'lSTNADP+v؏S @bNuvNADPHS(uNxTb P422V22-4 g:girR㉧Nug IeTϑ'YSNR N*N6kP423 V22-5 1 \'YRP[R:N\RP[USCQ ʑ>evϑ N)R(u0 2 \Ty\RP[USCQR:NqQ TvM㉧NirYNpCoA Nu؏SRNADPHT\ϑATP0 3 YNpCoAǏTCA[hQ'lSbCO2 1 Nv5uP[~'lSxxSNu'YϑvATP0nZ" ">  l!  /   9 50 R0TbS N Te_ USTN"vS z^Sc RgN"vS [ T]vvSb6R OS TSb6R @`" "N[ 4,{N N" N"/fuirgۏSǏ z-N :N^sXvSSv b_bvNy^R0ۏS z^ؚvuir vQN"v:g6R YBg0[U0 uirN"(W N*N4ls^ NۏL sSv4ls^0~ހ4ls^0Y~ހteSO4ls^^y~0o } 0vT~ހ4ls^v /fgW,gve_ :NNRuir@bqQ g0T#"#  l^y~4ls^ R ir o }4ls^ i ir ~ހ4ls^ v4ls^ US~ހuir77 7 .^y~teSOv0gؚ~v0 o }S^y~c6R0,{N~0 vSYv0W,gv0,{ N~0 v4ls^vv;m'`vSo;m0+RgHe^0qQNOp Tv+TϑWVhc N0 v4ls^v v4ls^v ;NǏv[MOv:SWS0v;m'`v0v+Tϑv ُ N*NebۏL0Vc""*  10 v[MOv:SWS v(W~ހQ gN[v^@\T[MO0PS N TN"_vv{| __R+R~bTyYvSO|0YvSO|X[(WNN[vN~ހ~g:SW-N bX[(WNހ(-N ُysay:Nvv:SWS0 RSm)He^ 2bkr^pb ON0 t$YvSO|(W~ހ-N:SWS :Nv4ls^vR N g)RagN,OgNV }SNNN0Wq_T~ހQgNRvv;m'` Nq_TvQ[MOvv;m'`0 u$dkY v[MOv:SWS O[N^irTR(W~ހhVQNwv[Sm) )RN(W~ހ@\VQ_ۏLT*NN"S^0 ;NN"_v|(W~ހQvR^ ހ(|u |STb xxb|_ xTb Rˆ}v(Tb0 ~|SOx'lS Nx_s |T8T 'lSxxS0 ~ހ8h8hxvTb0Op0 Q(Qˆ}v(Tb xTb0 ހ(T~|SO|_u ƀVTb nvSOYy4lv@s""f%M 20 v;m'`v e_vSvo;m pH9eS Yñv0pH7 e;m'`0pH5 ;m'`ؚ0 T]v qQNOp SuirSOQg͑ yrp_0upOe peypeRS[b0 1 0 qQNOpT~T>e'Y P430V22-14 xxS/1xxS zpS/1zpS 2 0 MRTS MR^ir[v;m'`v N,/fMRo;m FO_NS/fMRb6R0S_^irSm^ǏؚeSMQN"_vǏRb$cTNirv'YϑTb YؚSm^vYNpCoA/fYNpCoASvv+Rgb6RBR SMQNNxUSpCoA'YϑTb0 SNir[v;m'`v N,/fSb6R FO_N gSo;m0[""""#iq& aSb6R USNSb6R YNSb6R S_^R~NirSm^y/}ǏYe Ob6RRYS^vv;m'` Ote*NSO|S^^MNO0 b. z^Sb6R c. OS TSb6R d. /}ySb6R e. T]vSb6R f. So;mTMRo;m 3 0 So;m 4 0 MRo;m Y(W|u-N 1.6 Nxxg| ScؚTbNnxovv;m'` Rxxp_NnxlS:NNnx0 YS_Nnx N~YNpCoAۏeQTCAe Nnxy/} xxp_NnxlSbIpYNx TSTba.aT'VvU8hx0TbQv'VvU8hx So;mxxpNnxSv OۏIpYNxTb OTCA[IpYNxv0 30 vTbvWVhv vTb /fǏvϑvSSegcN"s0mZ" """Pb+7 N0 ~ހ4ls^v 1 c6R荜yP[Sm^CR^T5uMOh^ 2 c6R荜ir(Џ 3 :SSSm)\O(u 2bkr^pb ON 4 NvS~T SsQvNS~T Ǐ~TWTSnWvNSegvv;m'`0SsQv'YY/fN"_vsQ.vTv Y|u-Nv]|ov xxg|ov )v 3-xxul1"lv (lWxN"vGlu1"lv Tyr'lSvSNqQNOpvˆ}vov ˆ}vxxvI{0 N0 o }4ls^v|"""2% ,{N WVhv WVh gQ*N4ls^v t$lU_4ls^ u$l4ls^ v$R]4ls^ lU_TR]0ыTR] w$ˆ}v(;m'` vQ-NgsQ.v/ft$ WVhvc6R;NSu(WlU_4ls^,S8huir$\vQYdk0 e^ ^ N0 S8huirWVhv 10 ~P[!jW d~P[/fWVhvOSUSMO [+T g(WR N|_dk gsQvY*N~gWVSc6RMO c6RMO1u/TRP[Td~WV~b0 N*Nd~P[vhQWVcR(WNw vQ-N+TY*N~gWV lU_Nir/fUS*NYzSmRNA d~P[vc6RMOSSWVNirv0 20 ~bWWVT[WWV ~bvgbv SsXq_T\ ck8^N"agN Nh0Y|u㉄vv0 [v^Wv [ N TvuX[sX g N Tvh0YJSsN|v0)ZMZ#"#c"  ""t" "C&l  30 ckcTc (Wl gˆ}v(X[(We WV/fsQ필v ReQˆ}vT WV;m'`_/T dk:Nckc0 (Wl gˆ}vX[(We WV/fhv ReQˆ}vTWVh;m_sQ dk:Nc0 (Wckc-N ˆ}vy[ˆ}v0 (Wc-N ˆ}vy;Gˆ}v0 40 S8huir~gWVhv4yc6R!j_0 c[\O(u ^O;Gˆ}v㉻yDNA0 ;G\O(u ^O;Gˆ}v~TDNA0 P451V22-25 ckc[\O(u ^O[ˆ}v~TDNA0 ;G\O(u ^O[ˆ}v㉻yDNA0 VGr9-1 0hggku 0 P272 Z""v" ""p     50 'YFg̃sN|d~P[ Lacd~P[ ~gV: P453 V22-26 LacZ0LacY0LacA:N~gWV N8nO!k:Nd~WV0/TRP[TWVLacI0 S_~ހQe[irsN|bIPTG X[(We ;Oˆ}vNd~WV~T01uNd~WVN/TRP[ gN[ z^͑S YxNRNAZTv(W-10^R Nb_b_>e'`/TRP[ YTir0 S_~ހQ g[irsN|bIPTG X[(We [irN;Oˆ}v~T 9eS;Oˆ}vga OKNŏNd~WV N㉻y Neg0ُ7hRNAZTv1\N/TRP[~T v^b_b_>e'`/TRP[ YTir N _YlU_LacZYA~gWV0 VGr8-3 0Y[CNi` 0P 285 IPTG_NW--DkxNJSsN|[pa[ir [sN|d~P[Nug:_v[He^ /f:_[ir0\^Z" ""D     %#) &  60 r(lxd~P[trp vlU_c trpd~P[#TrpvTb 8^/f_>ev WVvNirO[sQ ُyc\O(uy:NS;OWvc0 t$E.coli trpd~P[ g5*N~gWV trpE-D-C-B-A0 u$(WtrpEv N8n g N*N:SktrpP-O-L trpL/fNk162bp^R lU_0RmRNA-Nb:NMR[^R [d~P[vlU_wc\O(u0 v$(WgrSO90R:S gtrpRWV x12.5kdv;Oˆ}vNW,NVZSOb__~T0RtrpO0 TrpPNN,S8hWV/TRP[N7h wQ g-10^RT-35^R -10^R[hQMONtrpPKNQ0 E.coli trpd~P[v~bSWVNirvR0 VGr E.coli wQ gTbTya.avR0(WYpe`Q N S g(WW{QW NO^Yna.ae MbSTbNua.a@b_{vv|0 S_~ހQTrpSm^ؚe TrpN;Oˆ}vtrpRWVNir ~T NO[wQ g;m'` N NtrpOWV~T sQlU_0 S_~ހQTrpSm^_NOe ;OGˆ}v NvTrp㉻yQeg O;Oˆ}v1Y;m v^1YSNtrpO~TvR _/TlU_0 VGr\Z"""p*    $   ! 70 trpd~P[vMR[^R trp mRNARP[Ne_YTb (WtrpEWV_YlU_KNMR 'YYpemRNAO\Pbku ُ/fV:NMR[^RtrpL [d~P[cS%cN͑\O(u0 trp mRNAvMR[^RSMR[0 ~gWV N8nwQ g/TRP[ d~WV MR[^R pQP[:S0 mRNA 5 z g162b vQ-N139*NgbMR[^R0MR[^R1u14*Na.avMR[04*NNe:SkT1*NpQP[~bkpgb0 pQP[MON~gWV N8nMR[:Sv~bkOS0V "" M   (." dMR[^Rvyrp t$MR[^RvgN:Sk[+TGC0>\ gN*N+T8*NUv:Sk fgb NOVNv~bkOS0(3:SN4:Sgb~bkOSvS9Y~g) u$1:ST2:Sgb,{N*NS9Y~g vQ-N1:SYN14*Na.avMR[^R-N0 v$3:SN2:S_Nb_bSN*NS9Y~g N S;bk3:SN4:Sb_b~bkS9Y~g0 w$MR[^RRNAxNk14a.avMR[ v^ gN~bk[xP[UGA 24 # hx$MR[^R-N v^RN*NTrp[xP[. (WmRNATbǏ z-N 1:SN2:SHQM[ R3:SN4:SM[,~bklU_. VGr: ;OTpQ:g6R }6q/f(WlU_4ls^ NۏL FO/f[Nv\O(u:g6R[hQ N T MRc6RlU_vwY Tc6RlU_wYT/f&T~~ NS0 (lWxTbd~P[MR[^R P454h22-2 u^: %N'}c6R WVhe^ ы4ls^L q($  N0 w8hWVhv X " "       v  &4(  4~ 4 s * $P  H 4 0޽h ? ̙33v  &8(  8~ 8 s *$P $ H 8 0޽h ? ̙33v  &<(  <~ < s *|1$P  H < 0޽h ? ̙33v  &@(  @~ @ s *TK$P $ H @ 0޽h ? ̙33v  &D(  D~ D s *|c$P $ H D 0޽h ? ̙33v  &H(  H~ H s *eP $ H H 0޽h ? ̙33v  & L(  L~ L s *eP e H L 0޽h ? ̙33v  &0P(  P~ P s *eP $ H P 0޽h ? ̙33v  &@T(  T~ T s * eP e H T 0޽h ? ̙33v  &PX(  X~ X s *-eP e H X 0޽h ? ̙33v  &`\(  \~ \ s *:eP e H \ 0޽h ? ̙33v  &p`(  `~ ` s *LeP e H ` 0޽h ? ̙33v  &d(  d~ d s *`feP e H d 0޽h ? ̙33v  &h(  h~ h s *eP e H h 0޽h ? ̙33v  &l(  l~ l s *eP e H l 0޽h ? ̙33v  &p(  p~ p s * P e H p 0޽h ? ̙33v  &t(  t~ t s * P e H t 0޽h ? ̙33v  &x(  x~ x s * P e H x 0޽h ? ̙33v  &|(  |~ | s * P   H | 0޽h ? ̙33v  &(  ~  s *X= P   H  0޽h ? ̙33v  &(  ~  s *  P   H  0޽h ? ̙33v  &(  ~  s * P   H  0޽h ? ̙33z *" (   v  3 A?055,$D 0  B96T4,$D 0 ^ WVhc$'B H  0޽h ? ̙33rlC`"S$%O'(K*+G-.C01?34;6779:3<=/?@+B  +F$<1U(   +ech Word.Document.80"Microsoft Word ech0VGr Word.Pict  !"#$%&'()*,-./0123456789:;<=>?@Oh+'0 `h  PowerPoint ʾĸczmczm2mMicrosoft PowerPointP@@Z@o@Pdev1uADPUclЏ0&TR 1uNpb__ceS0RhTVsX-N0" "",""I""  &j  $40 R:NTbcO؏SRTϑ ir(N"vW,geu(WNubATP0؏SRT~gUSCQ(uNSOQuirTb0 NADPHNN(uN؏S'`uirTb NADHTFADH2;NR/fǏ|T8TNuATP0 ATPegn1 ^ir4ls^xxS02 ~r iirTIQT~̃vIQTxxS03 |T8Tv'lSxxS0 NADPHegn 1 iirIQT5uP[ O 2 xx b|_ 3 YNpCoA1u~|SOly0R~ހ(e4O gNADHv'lSTNADP+v؏S @bNuvNADPHS(uNxTb P422V22-4 g:girR㉧Nug IeTϑ'YSNR N*N6kP423 V22-5 1 \'YRP[R:N\RP[USCQ ʑ>evϑ N)R(u0 2 \Ty\RP[USCQR:NqQ TvM㉧NirYNpCoA Nu؏SRNADPHT\ϑATP0 3 YNpCoAǏTCA[hQ'lSbCO2 1 Nv5uP[~'lSxxSNu'YϑvATP0nZ" ">  l!  /   9 50 R0TbS N Te_ USTN"vS z^Sc RgN"vS [ T]vvSb6R OS TSb6R @`" "N[ 4,{N N" N"/fuirgۏSǏ z-N :N^sXvSSv b_bvNy^R0ۏS z^ؚvuir vQN"v:g6R YBg0[U0 uirN"(W N*N4ls^ NۏL sSv4ls^0~ހ4ls^0Y~ހteSO4ls^^y~0o } 0vT~ހ4ls^v /fgW,gve_ :NNRuir@bqQ g0T#"#  l^y~4ls^ R ir o }4ls^ i ir ~ހ4ls^ v4ls^ US~ހuir77 7 .^y~teSOv0gؚ~v0 o }S^y~c6R0,{N~0 vSYv0W,gv0,{ N~0 v4ls^vv;m'`vSo;m0+RgHe^0qQNOp Tv+TϑWVhc N0 v4ls^v v4ls^v ;NǏv[MOv:SWS0v;m'`v0v+Tϑv ُ N*NebۏL0Vc""*  10 v[MOv:SWS v(W~ހQ gN[v^@\T[MO0PS N TN"_vv{| __R+R~bTyYvSO|0YvSO|X[(WNN[vN~ހ~g:SW-N bX[(WNހ(-N ُysay:Nvv:SWS0 RSm)He^ 2bkr^pb ON0 t$YvSO|(W~ހ-N:SWS :Nv4ls^vR N g)RagN,OgNV }SNNN0Wq_T~ހQgNRvv;m'` Nq_TvQ[MOvv;m'`0 u$dkY v[MOv:SWS O[N^irTR(W~ހhVQNwv[Sm) )RN(W~ހ@\VQ_ۏLT*NN"S^0 ;NN"_v|(W~ހQvR^ ހ(|u |STb xxb|_ xTb Rˆ}v(Tb0 ~|SOx'lS Nx_s |T8T 'lSxxS0 ~ހ8h8hxvTb0Op0 Q(Qˆ}v(Tb xTb0 ހ(T~|SO|_u ƀVTb nvSOYy4lv@s""f%M 20 v;m'`v e_vSvo;m pH9eS Yñv0pH7 e;m'`0pH5 ;m'`ؚ0 T]v qQNOp SuirSOQg͑ yrp_0upOe peypeRS[b0 1 0 qQNOpT~T>e'Y P430V22-14 xxS/1xxS zpS/1zpS 2 0 MRTS MR^ir[v;m'`v N,/fMRo;m FO_NS/fMRb6R0S_^irSm^ǏؚeSMQN"_vǏRb$cTNirv'YϑTb YؚSm^vYNpCoA/fYNpCoASvv+Rgb6RBR SMQNNxUSpCoA'YϑTb0 SNir[v;m'`v N,/fSb6R FO_N gSo;m0[""""#iq& aSb6R USNSb6R YNSb6R S_^R~NirSm^y/}ǏYe Ob6RRYS^vv;m'` Ote*NSO|S^^MNO0 b. z^Sb6R c. OS TSb6R d. /}ySb6R e. T]vSb6R f. So;mTMRo;m 3 0 So;m 4 0 MRo;m Y(W|u-N 1.6 Nxxg| ScؚTbNnxovv;m'` Rxxp_NnxlS:NNnx0 YS_Nnx N~YNpCoAۏeQTCAe Nnxy/} xxp_NnxlSbIpYNx TSTba.aT'VvU8hx0TbQv'VvU8hx So;mxxpNnxSv OۏIpYNxTb OTCA[IpYNxv0 30 vTbvWVhv vTb /fǏvϑvSSegcN"s0mZ" """Pb+7 N0 ~ހ4ls^v 1 c6R荜yP[Sm^CR^T5uMOh^ 2 c6R荜ir(Џ 3 :SSSm)\O(u 2bkr^pb ON 4 NvS~T SsQvNS~T Ǐ~TWTSnWvNSegvv;m'`0SsQv'YY/fN"_vsQ.vTv Y|u-Nv]|ov xxg|ov )v 3-xxul1"lv (lWxN"vGlu1"lv Tyr'lSvSNqQNOpvˆ}vov ˆ}vxxvI{0 N0 o }4ls^v|"""2% ,{N WVhv WVh gQ*N4ls^v t$lU_4ls^ u$l4ls^ v$R]4ls^ lU_TR]0ыTR] w$ˆ}v(;m'` vQ-NgsQ.v/ft$ WVhvc6R;NSu(WlU_4ls^,S8huir$\vQYdk0 e^ ^ N0 S8huirWVhv 10 ~P[!jW d~P[/fWVhvOSUSMO [+T g(WR N|_dk gsQvY*N~gWVSc6RMO c6RMO1u/TRP[Td~WV~b0 N*Nd~P[vhQWVcR(WNw vQ-N+TY*N~gWV lU_Nir/fUS*NYzSmRNA d~P[vc6RMOSSWVNirv0 20 ~bWWVT[WWV ~bvgbv SsXq_T\ ck8^N"agN Nh0Y|u㉄vv0 [v^Wv [ N TvuX[sX g N Tvh0YJSsN|v0)ZMZ#"#c"  ""t" "C&l  30 ckcTc (Wl gˆ}v(X[(We WV/fsQ필v ReQˆ}vT WV;m'`_/T dk:Nckc0 (Wl gˆ}vX[(We WV/fhv ReQˆ}vTWVh;m_sQ dk:Nc0 (Wckc-N ˆ}vy[ˆ}v0 (Wc-N ˆ}vy;Gˆ}v0 40 S8huir~gWVhv4yc6R!j_0 c[\O(u ^O;Gˆ}v㉻yDNA0 ;G\O(u ^O;Gˆ}v~TDNA0 P451V22-25 ckc[\O(u ^O[ˆ}v~TDNA0 ;G\O(u ^O[ˆ}v㉻yDNA0 VGr9-1 0hggku 0 P272 Z""v" ""p     50 'YFg̃sN|d~P[ Lacd~P[ ~gV: P453 V22-26 LacZ0LacY0LacA:N~gWV N8nO!k:Nd~WV0/TRP[TWVLacI0 S_~ހQe[irsN|bIPTG X[(We ;Oˆ}vNd~WV~T01uNd~WVN/TRP[ gN[ z^͑S YxNRNAZTv(W-10^R Nb_b_>e'`/TRP[ YTir0 S_~ހQ g[irsN|bIPTG X[(We [irN;Oˆ}v~T 9eS;Oˆ}vga OKNŏNd~WV N㉻y Neg0ُ7hRNAZTv1\N/TRP[~T v^b_b_>e'`/TRP[ YTir N _YlU_LacZYA~gWV0 VGr8-3 0Y[CNi` 0P 285 IPTG_NW--DkxNJSsN|[pa[ir [sN|d~P[Nug:_v[He^ /f:_[ir0\^Z" ""D     %#) &  60 r(lxd~P[trp vlU_c trpd~P[#TrpvTb 8^/f_>ev WVvNirO[sQ ُyc\O(uy:NS;OWvc0 t$E.coli trpd~P[ g5*N~gWV trpE-D-C-B-A0 u$(WtrpEv N8n g N*N:SktrpP-O-L trpL/fNk162bp^R lU_0RmRNA-Nb:NMR[^R [d~P[vlU_wc\O(u0 v$(WgrSO90R:S gtrpRWV x12.5kdv;Oˆ}vNW,NVZSOb__~T0RtrpO0 TrpPNN,S8hWV/TRP[N7h wQ g-10^RT-35^R -10^R[hQMONtrpPKNQ0 E.coli trpd~P[v~bSWVNirvR0 VGr E.coli wQ gTbTya.avR0(WYpe`Q N S g(WW{QW NO^Yna.ae MbSTbNua.a@b_{vv|0 S_~ހQTrpSm^ؚe TrpN;Oˆ}vtrpRWVNir ~T NO[wQ g;m'` N NtrpOWV~T sQlU_0 S_~ހQTrpSm^_NOe ;OGˆ}v NvTrp㉻yQeg O;Oˆ}v1Y;m v^1YSNtrpO~TvR _/TlU_0 VGr\Z"""p*    $   ! 70 trpd~P[vMR[^R trp mRNARP[Ne_YTb (WtrpEWV_YlU_KNMR 'YYpemRNAO\Pbku ُ/fV:NMR[^RtrpL [d~P[cS%cN͑\O(u0 trp mRNAvMR[^RSMR[0 ~gWV N8nwQ g/TRP[ d~WV MR[^R pQP[:S0 mRNA 5 z g162b vQ-N139*NgbMR[^R0MR[^R1u14*Na.avMR[04*NNe:SkT1*NpQP[~bkpgb0 pQP[MON~gWV N8nMR[:Sv~bkOS0V "" M   (." dMR[^Rvyrp t$MR[^RvgN:Sk[+TGC0>\ gN*N+T8*NUv:Sk fgb NOVNv~bkOS0(3:SN4:Sgb~bkOSvS9Y~g) u$1:ST2:Sgb,{N*NS9Y~g vQ-N1:SYN14*Na.avMR[^R-N0 v$3:SN2:S_Nb_bSN*NS9Y~g N S;bk3:SN4:Sb_b~bkS9Y~g0 w$MR[^RRNAxNk14a.avMR[ v^ gN~bk[xP[UGA 24 # hx$MR[^R-N v^RN*NTrp[xP[. (WmRNATbǏ z-N 1:SN2:SHQM[ R3:SN4:SM[,~bklU_. VGr: ;OTpQ:g6R }6q/f(WlU_4ls^ NۏL FO/f[Nv\O(u:g6R[hQ N T MRc6RlU_vwY Tc6RlU_wYT/f&T~~ NS0 (lWxTbd~P[MR[^R P454h22-2 u^: %N'}c6R WVhe^ ы4ls^L q($  N0 w8hWVhv X " "       r0   i$<